Survival of apurinic SV40 DNA in the d-complementation group of xeroderma pigmentosum. |
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Authors: | R D Kudrna J Smith S Linn E E Penhoet |
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Institution: | Department of Biochemistry, University of California, Berkeley, CA 94720, U.S.A. |
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Abstract: | The survival of depurinated Form I SV40 DNA was studied in normal human fibroblasts and in D-complementation Xeroderma pigmentosum (XP) fibroblasts. Survival was measured with an infective center assay. Heat-acid and methyl methanesulfonate (MMS) were used as depurinating agents. After 3 hrs of depurination by heat--acid treatment, infectivity in normal cells was less than 15% of the controls compared to more than 50% for the XP D cell strains. Similar results were obtained with MMS-treated DNA. These results are contrary to expectation since apurinic endonuclease activity, which is presumed to be involved in the repair of apurinic sites, is much lower in XP D cell strains than in normal cell strains. Our results indicate that another mechanism for the repair of apurinic sites could exist. |
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Keywords: | To whom reprint requests should be addressed |
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