| 2,4 -D对甘薯体细胞胚胎发生的调控 |
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| 引用本文： | 崔红 丁群星 桂耀林 郭仲琛. 2,4 -D对甘薯体细胞胚胎发生的调控[J]. 植物学报, 1999, 16(4): 411-415 |
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| 作者姓名： | 崔红 丁群星 桂耀林 郭仲琛 |
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| 作者单位： | 1(厦门大学生物系 厦门 361005） 2(中国科学院植物研究所 北京 100093） |
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| 摘 要： | 将来源于‘徐薯18’叶片的胚性愈伤组织，接种在含有不同2,4-D浓度的液体MS培养基中进行悬浮培养，悬浮细胞表现出不同的形态结构、分裂方式和发育途径：2,4-D浓度为1 mg/L时，细胞均等分裂，增殖迅速；不含2,4-D时，细胞多进行不均等分裂，并发育成体细胞胚。不同2,4-D浓度中培养的悬浮细胞，其胞外过氧化物同工酶谱及其随时间变化的方式有很大差异，并与细胞的生长、发育过程密切相关。
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| 关 键 词： | 甘薯 体胚发生 悬浮培养 过氧化物同工酶 |
| 收稿时间： | 1998-04-13 |
| 修稿时间： | 1998-07-06 |
2,4-D Regulated Somatic Em bryogenesis of Sweet Potato |
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| CUI Hong,DING Qun-Xing,GUI Yao-Lin and GUO Zhong-Chen. 2,4-D Regulated Somatic Em bryogenesis of Sweet Potato[J]. Bulletin of Botany, 1999, 16(4): 411-415 |
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| Authors: | CUI Hong DING Qun-Xing GUI Yao-Lin GUO Zhong-Chen |
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| Affiliation: | 1(Department of Biology, Xiamen University, Xiamen 361005) 2(Institute of Botany, The Chinese Academy Sciences, Beijing 100093) |
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| Abstract: | Divergences were discovered in cell morphology, mode of cell division and passage of cell differentiation, when embryogenic calli induced from leaves of ‘Xushu 18’ sweet potato were suspension-cultured in modified MS medium with different concentrations of 2, 4-D. Cells grewquickly and divided equally in medium containing 1 mg/L 2,4-D. When 2,4-D was deleted, the cells divided unequally and developed into embryos. Extracellular pemxidase isozyme patterns changed greatly during suspension culture with different concentrations of 2,4-D and were closely related to cell development process. |
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| Keywords: | ; |
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