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2,4 -D对甘薯体细胞胚胎发生的调控
引用本文:崔红 丁群星 桂耀林 郭仲琛. 2,4 -D对甘薯体细胞胚胎发生的调控[J]. 植物学报, 1999, 16(4): 411-415
作者姓名:崔红 丁群星 桂耀林 郭仲琛
作者单位:1(厦门大学生物系 厦门 361005) 2(中国科学院植物研究所 北京 100093)
摘    要:将来源于‘徐薯18’叶片的胚性愈伤组织,接种在含有不同2,4-D浓度的液体MS培养基中进行悬浮培养,悬浮细胞表现出不同的形态结构、分裂方式和发育途径:2,4-D浓度为1 mg/L时,细胞均等分裂,增殖迅速;不含2,4-D时,细胞多进行不均等分裂,并发育成体细胞胚。不同2,4-D浓度中培养的悬浮细胞,其胞外过氧化物同工酶谱及其随时间变化的方式有很大差异,并与细胞的生长、发育过程密切相关。

关 键 词:甘薯  体胚发生  悬浮培养  过氧化物同工酶
收稿时间:1998-04-13
修稿时间:1998-07-06

2,4-D Regulated Somatic Em bryogenesis of Sweet Potato
CUI Hong,DING Qun-Xing,GUI Yao-Lin and GUO Zhong-Chen. 2,4-D Regulated Somatic Em bryogenesis of Sweet Potato[J]. Bulletin of Botany, 1999, 16(4): 411-415
Authors:CUI Hong  DING Qun-Xing  GUI Yao-Lin  GUO Zhong-Chen
Affiliation:1(Department of Biology, Xiamen University, Xiamen 361005) 2(Institute of Botany, The Chinese Academy Sciences, Beijing 100093)
Abstract:Divergences were discovered in cell morphology, mode of cell division and passage of cell differentiation, when embryogenic calli induced from leaves of ‘Xushu 18’ sweet potato were suspension-cultured in modified MS medium with different concentrations of 2, 4-D. Cells grewquickly and divided equally in medium containing 1 mg/L 2,4-D. When 2,4-D was deleted, the cells divided unequally and developed into embryos. Extracellular pemxidase isozyme patterns changed greatly during suspension culture with different concentrations of 2,4-D and were closely related to cell development process.
Keywords:;
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