Factors Influencing Alanine Aminotransferase Activity in Leaves of Lolium temulentumL.: II. EFFECTS OF GROWTH REGULATORS AND PROTEIN BIOSYNTHESIS INHIBITORS |
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Authors: | HEDLEY, C. L. STODDART, J. L. |
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Abstract: | Effects of kinetin (K), gibberellin A3 (GA3), and 2-(chloroethyl)-trimethylammoniumchloride (CCC) on levels of alanine aminotransferase (GPT) andrates of protein synthesis were studied with both intact plantsand isolated leaf segments of Lolium temulentum L. In intactplants CCC stimulated and CA3 reduced GPT activity, the effectsbsing much greater in 8.h than in 16-h photoporiods. CCC showedmaximum stimulatory effects at 102 M and K at 5 x 105M. No effect of GA3 could be demonstrated with concentrationsup to 104M. Both K and CCC retarded GPT decline in leafsections, the latter without associated effects upon pigmentbreakdown. Cycloheximide was highly effective in reducing proteinsynthesis in leaf sections. A close correlation between rateof protein synthesis and GPT activity was found over an inhibitorconcentration range from 106 to 104 M. The resultsare discussed in terms of possible methods of in vivo regulationof GPT activity. |
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