Neocarzinostatin: controlled release of chromophore and its interaction with DNA |
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| Authors: | Pankaj Ganguly Nancy G. Fossett |
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| Affiliation: | 1. Department of Hematology, St. Jude Children''s Research Hospital Memphis, Tennessee 38101 USA;2. the Department of Biochemistry, University of Tennessee, Memphis, Tennessee 38101 USA |
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| Abstract: | A nonagglutinating derivative of wheat germ agglutinin has been prepared that binds to platelets and precipitates an antibody to the lectin. Platelets treated with this inactive derivative released serotonin when exposed to bivalent F(ab′)2, but not monovalent Fab, fragments of the lectin antibody. Bridging of platelet-bound Fab by an antibody again induced secretion. The F(ab′)2 or Fab fragments plus IgG, without the derivative, did not induce secretion. This secretion was not affected by indomethacin showing a direct activation of platelets. Platelets treated with con A followed by F(ab′)2 to con A did not secrete. In addition, lentil lectin failed to release platelet serotonin. The receptors of the lectin derivative are mobile on the platelet surface and their redistribution may lead to secretion. |
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| Keywords: | NCS neocarzinostatin SSC standard saline citrate CD circular dichroism Tris Tris(hydroxymethyl)-aminomethan |
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