Inheritance and structure of foreign DNA in progenies of transgenic tobacco obtained by direct gene transfer |
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Authors: | H Morota H Uchimiya |
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Institution: | (1) Institute of Biological Sciences, University of Tsukuba, Tsukuba-city, 305 Ibaraki, Japan |
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Abstract: | Summary One of the transformed tobacco plants obtained by direct DNA transformation possessed two marker genes, a chimeric aminoglycoside phosphotransferase and nopaline synthase genes. Selfed progenies of this plant (T3-d) showed stable inheritance of these two genes. The minimum size of foreign DNA integrated into tobacco genome was estimated to be 5.4 kbp. A deleted nopaline synthase gene co-existed with an intact gene. The linkage analysis indicated that two transformants, T1-b and T3-c, possessed foreign DNA inserted in different chromosomes or in different sites of the same chromosome that recombine freely. |
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Keywords: | Tobacco Direct DNA transformation Insertion site Linkage analysis |
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