Dipeptidyl peptidase with strict substrate specificity of an anaerobic periodontopathogen Porphyromonas gingivalis |
| |
Authors: | Fujimura Setsuo Hirai Kaname Shibata Yukinaga |
| |
Institution: | Department of Oral Microbiology, Matsumoto Dental University, Shiojiri-Shi, Nagano-Ken 399-0781, Japan. fujimura@po.mdu.ac.jp |
| |
Abstract: | A dipeptidyl peptidase which hydrolyzed Xaa-Ala-p-nitroanilide was purified to homogeneity by sequential procedures including ammonium sulfate precipitation, ion-exchange chromatography, hydrophobic interaction chromatography, gel filtration and isoelectric focusing from the cell extract of Porphyromonas gingivalis. The purified enzyme hydrolyzed p-nitroanilide derivatives of Lys-Ala, Ala-Ala, and Val-Ala, but not Xaa-Pro. Enzyme activity was maximum at neutral pHs. Its molecular mass was 64 kDa with an isoelectric point of 5.7. The enzyme belonged to the family of serine peptidases. |
| |
Keywords: | Wood-rotting fungus Dioxin 2 7-Dichlorodibenzo-p-dioxin Degradation |
本文献已被 PubMed 等数据库收录! |
|