Streptomonospora algeriensis sp. nov., a halophilic actinomycete isolated from soil in Algeria

A halophilic actinomycete strain, designated H27^T, was isolated from a soil sample collected from a hypersaline habitat in Djelfa Province (North-Central Algeria), and then investigated using a polyphasic taxonomic approach. The strain was observed to produce poor aerial mycelium, which formed short chains of oval to cylindrical-shaped spores at maturity, and non fragmented substrate mycelium. The optimum NaCl concentration for growth was found to be 10–15 % (w/v) and the optimum growth temperature and pH were found to be 28–37 °C and 6–7, respectively. The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinones of strain H27^T were identified as MK-11 (H₄) and MK-10 (H₆). The major fatty acids were found to be iso-C_16:0, anteiso-C_17:0, 10 methyl C_17:0 and 10 methyl C_16:0. The diagnostic phospholipids detected were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. The chemotaxonomic properties of strain H27^T are consistent with those shared by members of the genus Streptomonospora. 16S rRNA gene sequence analysis indicated that strain H27^T is most closely related to Streptomonospora alba DSM 44588^T (98.8 %) and Streptomonospora flavalba DSM 45155^T (98.7 %) whereas the DNA–DNA relatedness values between strain H27^T and the two type strains were 17.1 and 57.9 %, respectively. Based on the combined genotypic and phenotypic evidence, it is proposed that strain H27^T should be classified as representative of a novel species, for which the name Streptomonospora algeriensis sp. nov. is proposed. The type strain is H27^T (=DSM 45604^T =CCUG 63369^T =MTCC 11563^T).