Development and validation of a robust,breeder-friendly molecular marker for the vc- locus in faba bean |
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Authors: | Hamid Khazaei Randy W. Purves Melody Song Robert Stonehouse Kirstin E. Bett Frederick L. Stoddard Albert Vandenberg |
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Affiliation: | 1.Department of Plant Sciences,University of Saskatchewan,Saskatoon,Canada;2.Evan Hardy Collegiate Institute,Saskatoon,Canada;3.Department of Agricultural Sciences, Viikki Plant Science Centre,University of Helsinki,Helsinki,Finland |
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Abstract: | Faba bean (Vicia faba L.) is a valuable feed and food crop with potential for development globally as a staple protein crop. Its consumption is limited by the anti-nutritional factors vicine and convicine (v-c) in its seeds. A single gene (vc - ) confers the low v-c phenotype in faba bean. Time-consuming and laborious quantitative chemical analysis is currently used in breeding programs to detect v-c concentration. Molecular markers within or linked to the vc - gene could facilitate rapid and cost-effective screening of early generation breeding populations for low v-c concentration. The large and complex faba bean genome has been an impediment to the progress of development of molecular breeding strategies. Here, we report a high-throughput low-cost KASP (Kompetitive Allele Specific PCR) marker for low v-c concentration in faba bean. The KASP assay successfully distinguished low and high v-c lines of faba bean. This marker is a significant and valuable molecular tool for faba bean breeding programs aiming to reduce v-c from faba beans worldwide. |
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