Towards new sources of resistance to the currant-lettuce aphid (<Emphasis Type="Italic">Nasonovia ribisnigri</Emphasis>) |
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Authors: | Peter G Walley Gemma Hough Jonathan D Moore John Carder Marian Elliott Andrew Mead Julie Jones Graham Teakle Guy Barker Vicky Buchanan-Wollaston Paul Hand David Pink Rosemary Collier |
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Institution: | 1.Functional and Comparative Genomics, Institute of Integrative Biology,University of Liverpool,Liverpool,UK;2.Syngenta, Jealott’s Hill International Research Centre,Bracknell,UK;3.Earlham Institute,Norwich Research Park,Norwich,UK;4.School of Life Sciences, Warwick Crop Centre,The University of Warwick,Warwick,UK;5.Rothamsted Research,West Common,Harpenden,UK;6.Harper Adams University College,Newport,UK |
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Abstract: | Domesticated lettuce varieties encompass much morphological variation across a range of crop type groups, with large collections of cultivars and landrace accessions maintained in genebanks. Additional variation not captured during domestication, present in ancestral wild relatives, represents a potentially rich source of alleles that can deliver to sustainable crop production. However, these large collections are difficult and costly to screen for many agronomically important traits. In this paper, we describe the generation of a diversity collection of 96 lettuce and wild species accessions that are amenable to routine phenotypic analysis and their genotypic characterization with a panel of 682 newly developed expressed sequence tag (EST)-linked KASP? single nucleotide polymorphism (SNP) markers that are anchored to the draft Lactuca sativa genome assembly. To exemplify the utility of these resources, we screened the collection for putative sources of resistance to currant-lettuce aphid (Nasonovia ribisnigri) and carried out association analyses to look for potential SNPs linked to resistance. |
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