Partial purification of gibberellin oxidases from spinach leaves |
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Authors: | Gilmour S J Bleecker A B Zeevaart J A |
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Affiliation: | MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824. |
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Abstract: | Four enzyme activities catalyzing the following oxidative steps in the gibberellin (GA) biosynthetic pathway have been extracted from spinach (Spinacia oleracea L.) leaves after exposure to 8 long days: GA12 → GA53 → GA44 → GA19 → GA20. Two of these, GA53 oxidase and GA19 oxidase, were separable from the other two, GA44 oxidase and GA12 13-hydroxylase, by anion exchange high performance liquid chromatography (HPLC). Apparent molecular weights of the four enzymes as determined by gel filtration HPLC are: GA12 13-hydroxylase, 28,400; GA53 oxidase, 42,500; GA44 oxidase, 38,100; GA19 oxidase, 39,500. GA44 oxidase was purified approximately 100-fold in 0.3% yield by a combination of ammonium sulfate fractionation, anion exchange HPLC, phenyl-Sepharose chromatography and gel filtration HPLC. |
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