Nit-3, the structural gene of nitrate reductase in Neurospora crassa: nucleotide sequence and regulation of mRNA synthesis and turnover |
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Authors: | Patricia M. Okamoto Ying-Hui Fu George A. Marzluf |
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Affiliation: | (1) Department of Biochemistry, The Ohio State University, 43210 Columbus, OH, USA |
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Abstract: | Summary The nit-3 gene of the filamentous fungus Neurospora crassa encodes the enzyme nitrate reductase, which catalyzes the first reductive step in the highly regulated nitrate assimilatory pathway. The nucleotide sequence of nit-3 was determined and translates to a protein of 982 amino acid residues with a molecular weight of approximately 108 kDa. Comparison of the deduced nit-3 protein sequence with the nitrate reductase protein sequences of other fungi and higher plants revealed that a significant amount of homology exists, particularly within the three cofactor-binding domains for molybdenum, heme and FAD. The synthesis and turnover of the nit-3 mRNA were also examined and found to occur rapidly and efficiently under changing metabolic conditions. |
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Keywords: | Neurospora crassa Nitrate reductase Nitrate assimilaton nit-3 sequence |
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