| 中国稻瘟病菌的致病性和其DNA指纹图谱间关系的研究 |
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| 引用本文： | 朱衡,王久林,雷财林,凌忠专,朱立煌.中国稻瘟病菌的致病性和其DNA指纹图谱间关系的研究[J].菌物学报,1993,12(Z1):57-64. |
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| 作者姓名： | 朱衡 王久林 雷财林 凌忠专 朱立煌 |
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| 作者单位： | 中国科学院遗传所, 北京;中国农科院作物所, 北京;中国农科院作物所, 北京;中国农科院作物所, 北京;中国科学院遗传所, 北京 |
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| 摘 要： | 通过在稻瘟病菌Pyricularia oryza。基因组文库中的筛选,找到一个散布的并具有基因组特异性的层重复顺序POR6。本文报道用这一株针对6个日本菌株和26个中国北方菌株进行DNA指纹作图的结果。其中22个中国北方菌株按其杂交带型百分相似率被分成8个株系。一些在我们实验室保存的菌株用传统方法鉴定发现在转管过程中会发生致病性变异。当用POR6作探针与这些菌株NNA的EcoRV酶切片段杂交时,检测出它们的无性世代中出现数条EcoRV多态性片段。
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| 关 键 词： | 稻瘟病菌 DNA指纹图谱 转管培养中的毒性变异 |
RELATIONSHIP BETWEEN DNA FINGERPRINTS AND PATHOTYPE CHARACTERIATION OF CHINESE RICE BLAST ISOLATES (Pyricularia oryzae) |
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| Authors: | ZHU Heng WANG JU-lin LEI Cai-lin LING zhong-zhuan and ZHU Li-huang |
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| Institution: | Instituteo f Genetics, Academia Sinica, Beijing;Institute of Crop Breeding and Cultivation, CAAS, Beijing;Institute of Crop Breeding and Cultivation, CAAS, Beijing;Institute of Crop Breeding and Cultivation, CAAS, Beijing;Instituteo f Genetics, Academia Sinica, Beijing |
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| Abstract: | By selecting from a genomic library of rice blast fungus, Pyricularia oryzae, we have cloned a genome specific and dispersed repeated sequence named POR6, which can be used to fingerprint various kinds of rice blast pathotypes. In this study, 6 isolates from Japan and 26 isolates from northern China were analyzed using POR6 as a probe. Twentytwo of northern China isolates were assigned to 8 lineages by evaluating the percentage of similar hybridization bands (Sxy) of each pairwise isolates. Some blast isolates maintained in our laboratory were proven to be varied with subsequent culturing in their pathotypes identified by the traditional method. When POR6 was used as a probe, two isolates showed variations in EcoRV digested DNA fingerprints among their asexual generations. |
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| Keywords: | Rice Blast fungus DNA fingerprints Virulence Difference in subsequent culturing |
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