| 两种疱脐衣的核rDNA ITS2序列变异及其系统学意义 |
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| 引用本文： | 牛永春,魏江春.两种疱脐衣的核rDNA ITS2序列变异及其系统学意义[J].菌物学报,1993,12(Z1):25-29. |
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| 作者姓名： | 牛永春 魏江春 |
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| 作者单位： | 中国科学院微生物研究所真菌地衣系统学开放研究实验室, 北京;中国科学院微生物研究所真菌地衣系统学开放研究实验室, 北京 |
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| 基金项目： | The project supported by the special fund of the Committee for the Systematic Biology of the CAS. |
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| 摘 要： | 本文从分子系统学角度为石耳科两个疑难种的分类处理提供了佐证。真菌DNA是从淡腹疱脐衣及露西疱脐衣不含藻细胞的亚茶渍型子囊盘中提取后,用聚合酶链式反应(PCR)技术对于核中核糖体脱氧核酸((rDNA)的转录间区2 (ITS2)片段进行了扩增,并进行了核苷酸序列测定。在分子水平与形态特征以及化学与分布学相结合中进行了比较系统学分析。
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| 关 键 词： | 聚合酶链式反应 核糖体脱氧核酸 转录间区2 核苷酸序列测定 淡腹疱脐衣 露西疱脐衣 |
VARIATIONS IN ITS2 SEQUENCES OF NUCLEAR rDNA FROM TWO LASALLIA SPECIES AND THEIR SYSTEMATIC SIGNIFICANCE |
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| Authors: | NIU Yong-chun and WEI Jiang-chun |
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| Institution: | Systematic Mycology & Lichenology Laboratory Institute of Microbiology, Academia Sinica, Beijing, China;Systematic Mycology & Lichenology Laboratory Institute of Microbiology, Academia Sinica, Beijing, China |
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| Abstract: | The internal transcribed spacer 2 (ITS2) of nuclear rDNA was amplified by PCR from total DNA extracted from sublecanorine apothecia of Lasallia papulosa (Ach.) Llano and L. rossica Dombr. lacking algal cell, and their sequences were determined. A systematic analysis based on a combination of the molecular and morphological characters with chemical data and distribution patterns is given. |
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| Keywords: | PCR rDNA ITS2 sequence Lasallia papulosa L rossica |
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