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Isolation and characterization of fourteen polymorphic microsatellite markers in the viperine snake Natrix maura
Authors:Hugo Le Chevalier,Neus Marí  ‐Mena,Bel  n Carro,J  r  me G. Prunier,Coralie Bossu,Elodie Darnet,J  r  mie Souchet,Olivier Guillaume,Olivier Calvez,Romain Bertrand,Laurent Barthe,Gilles Pottier,Albert Martí  nez‐Sylvestre,Isabel Verdaguer‐Foz,Marc Mossoll‐Torres,Audrey Trochet,Fabien Aubret
Affiliation:Hugo Le Chevalier,Neus Marí‐Mena,Belén Carro,Jérôme G. Prunier,Coralie Bossu,Elodie Darnet,Jérémie Souchet,Olivier Guillaume,Olivier Calvez,Romain Bertrand,Laurent Barthe,Gilles Pottier,Albert Martínez‐Sylvestre,Isabel Verdaguer‐Foz,Marc Mossoll‐Torres,Audrey Trochet,Fabien Aubret
Abstract:Nineteen polymorphic microsatellite loci were identified and developed for Natrix maura. Polymorphism was assessed for 120 individuals sampled across four sampling sites from the French Pyrenees Mountains. The number of alleles per locus ranged from 3 to 15, and expected heterozygosity per locus ranged from 0.227 to 0.863. We tested for deviation from Hardy–Weinberg equilibrium and linkage disequilibrium and assessed the presence of null alleles for all loci, resulting in a selection of 14 high‐quality polymorphic markers. These markers will be extremely useful in identifying fine‐scale genetic structures and providing insight into conservation management plans of this species.
Keywords:   Natrix maura     polymorphic microsatellites  population genetics  viperine snake
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