Localization and properties of ATPase activity in pea stems and wheat coleoptiles |
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| Authors: | J L Hall A J Kinney A Dymott J R Thorpe and D A Brummell |
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| Institution: | (1) School of Biological Sciences, University of Sussex, Brighton, UK;(2) Present address: Department of Biology, The University, Building 44, SO9 5NH Southampton, UK;(3) Present address: ARC Letcombe Laboratory, OX12 9JT Wantage, Oxfordshire, UK |
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| Abstract: | Summary Microsomal fractions from wheat coleoptiles and pea stems contain a microsomal ATPase activity that requires divalent cations (Ca2+ is more effective than Mg2+) and shows further stimulation by KCl. The effects of added indoleacetic acid were inconclusive. Cytochemical studies on both species showed most pronounced staining for ATPase in the plasmalemma at pH 7.0. However, at pH 5.5, the coleoptile cells showed heaviest staining for ATPase in the endoplasmic reticulum and dictyosomes. The results are discussed with regard to the postulated role of ATPase activity in relation to proton pumping and plant cell elongation. |
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