Collagenase in the immunohistochemical demonstration of laminin,fibronectin and factor VII/RAg in nervous tissue after fixation |
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Authors: | A. Mauro A. Bertolotto I. Germano G. Giaccone M. T. Giordana A. Migheli D. Schiffer |
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Affiliation: | (1) II. Neurological Clinic, University of Turin, Via Cherasco 15, I-10126 Turin, Italy |
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Abstract: | Summary The effects of collagenase on the immunohistochemical demonstrability of laminin, fibronectin and Factor VIII/RAg in human nervous tissue have been studied. The influence of this, and other proteolytic enzymes such as pepsin and trypsin, has been investigated in relation to different fixatives. Collagenase gave better results with Carnoy fixed material than after formalin fixation; unlike trypsin and pepsin, it did not produce tissue digestion.Partially supported by CNR Special Project Control of Neoplastic Growth, Grant No 82.00410.96 |
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