Steroidogenesis in the zona glomerulosa of the adrenal cortex II. Distribution of cytochrome P-450 in the zona glomerulosa of the bovine adrenal cortex |
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| Authors: | Takashi Wakabayashi Chieko Kurono Masahisa Asano Hiroshi Kimura Takayuki Ozawa |
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| Institution: | (1) Department of Pathology, Nagoya City University Medical School, Mizuho-ku, Nagoya, Japan;(2) Department of Anatomy, Nagoya City University Medical School, Mizuho-ku, Nagoya, Japan;(3) Department of Biochemistry, Nagoya City University Medical School, Mizuho-ku, Nagoya, Japan;(4) Institute for Biochemistry, Nagoya University Faculty of Medicine, Showa-ku, Nagoya, Japan |
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| Abstract: | Microsomes were obtained from the zona glomerulosa of the bovine adrenal cortex. Contamination of microsomes with other cellular organelles was examined using various marker enzymes and the electron microscope. Distribution of cytochrome P-450 in the zona glomerulosa was studied using various fractions including microsomes, described above, and mitochondria. The amount of cytochrome P-450 in mitochondria and in microsomes was determined to be 0.73 and 0.32 nmol/mg protein, respectively. The CO difference spectrum was affected not only by the concentration of added deoxycholate but also by the incubation time after addition. Approximately 40–50% of cytochrome P-450 in the samples was converted to cytochrome P-420 within 20–30 sec of incubation with deoxycholate.The content of RNA, phospholipids, and cytochromeb
5 in microsomes obtained from the zona glomerulosa is also evaluated in comparison to that in microsomes obtained from the zona fasciculoreticularis. |
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