Human monocytes selectively bind to cells expressing the tumorigenic phenotype |
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Authors: | Horoyuki Shimizu Deborah Wyatt Rebecca D. Knowles Corazon D. Bucana Eric J. Stanbridge Eugenie S. Kleinerman |
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Affiliation: | (1) Department of Pediatrics, The University of Texas, M. D. Anderson Hospital and Tumor Institute at Houston, 77030 Houston, TX;(2) Department of Cell Biology, The University of Texas, M. D. Anderson Hospital and Tumor Institute at Houston, 1515 Holcombe Blvd., Box 173, 77030 AAA Houston, TX;(3) Department of Microbiology and Molecular Genetics, University of California, 92717 Irvine, CA, USA |
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Abstract: | Summary The characteristics of the binding of human monocytes to tumor cells were studied by a newly developed microassay. First, we determined the kinetics and optimal conditions of the binding. Monocytes recognized and bound to tumor cells very rapidly within 10–20 min of cellular interaction. Binding was also more efficient at 37°C suggesting that active metabolism of monocytes is required. Second, we determined that selective binding of monocytes to cells with tumorigenic phenotypes occurs. For this purpose, lymphocytic leukemia cell lines versus normal lymphocytes, and tumorigenic versus nontumorigenic hybrids from the same parental lines were compared as the targets of the binding assay. In both cases, neoplastic cells were selectively bound by monocytes. Although tumor cells were bound rapidly and selectively by monocytes, initial recognition and binding did not necessarily lead to subsequent tumor cell lysis. This is based on the observation that some tumorigenic parental and hybrid lines were avidly bound by monocytes yet not subsequently killed in a cytotoxicity assay.This work was supported in part by a grant from the National Institutes of Health CA42992 and a grant from the Kleberg foundationAbbreviations used: [125I]IdUrd [125I]iododeoxyuridine; rIFN-, recombinant human interferon ; IL-1, interleukin 1; rTNF, recombinant human tumor necrosis factor |
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