Isolation and characterization of polymorphic microsatellite loci from black snapper Sebastodes fuscescens (Houttuyn) |
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| Authors: | Jing-Feng Yang Li-Li Zhao Chang-Wei Shao Xiang-Shan Ji Xiao-Lin Liao Song-Lin Chen |
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| Institution: | (1) College of Marine Life Sciences, Ocean University of China, 266003 Qingdao, China;(2) Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, 266071 Qingdao, China; |
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| Abstract: | Black snapper Sebastodes fuscescens (Houttuyn) is an economocally important species. Fourty-five microsatellite loci were isolated from an enriched genomic library
of S. fuscescens. Ten of these loci were polymorphic in a test population with alleles per locus ranging from 2 to 5, and observed and expected
heterozygosities per locus from 0.21 to 1.00 and from 0.19 to 0.86, respectively. Three loci significantly deviated from Hardy–Weinberg
equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs of loci. These polymorphic
microsatellite loci would be useful for investigating genetic diversity of S. fuscescens and other related species. |
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