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Modulation of neuropeptide Y and Y1 receptor expression in the amygdala by fluctuations in the brain content of neuroactive steroids during ethanol drinking discontinuation in Y1R/LacZ transgenic mice
Authors:Eva Carola  Mele Paolo  Collura Devis  Nai Antonella  Pisu Maria Giuseppina  Serra Mariangela  Biggio Giovanni
Affiliation:Dipartimento di Anatomia, Farmacologia e Medicina Legale, Sezione di Farmacologia, Universitàdi Torino, Torino, Italy;
Neuroscience Institute of Torino, Universitàdi Torino, Torino, Italy;
Dipartimento di Biologia Sperimentale, Sezione di Neuroscienze, Universitàdi Cagliari, Cagliari, Italy;
C. N. R. Istituto di Neuroscienze, Sezione di Cagliari, Cagliari, Italy;
Centro di Eccellenza per la Neurobiologia della Dipendenza, Universitàdi Cagliari, Cagliari, Italy
Abstract:Previous studies have shown that GABAergic neuroactive steroids increase Y1 receptor (Y1R) gene expression in the amygdala of Y 1 R / LacZ transgenic mice, harbouring the murine Y1R gene promoter linked to a LacZ reporter gene. As ethanol is known to increase GABAergic neuroactive steroids, we investigated the relationship between fluctuations in the brain content of neuroactive steroids induced by chronic voluntary ethanol consumption or ethanol discontinuation and both the level of neuropeptide Y (NPY) immunoreactivity and Y1R gene expression in the amygdala of Y 1 R / LacZ transgenic mice. Ethanol discontinuation (48 h) after voluntary consumption of consecutive solutions of 3%, 6%, 10% and 20% (v/v) ethanol over 4 weeks produced an anxiety-like behaviour as measured by elevated plus maze. Voluntary ethanol intake increased the cerebrocortical concentration of the progesterone metabolite 3α-hydroxy-5α-pregnan-20-one (3α,5α-TH PROG) that returned to control level 48 h after discontinuation of ethanol intake. Ethanol discontinuation significantly decreased NPY immunoreactivity and concomitantly increased Y 1 R / LacZ transgene expression in the amygdala, whereas chronic ethanol intake failed to affect these parameters. The 5α-reductase inhibitor finasteride prevented both the increase in the cerebrocortical concentration of 3α,5α-TH PROG apparent after 4 weeks of ethanol intake and the changes in NPY immunoreactivity and transgene expression induced by ethanol discontinuation. Data suggest that 3α,5α-TH PROG plays an important role in the changes in NPY–Y1R signalling in the amygdala during ethanol discontinuation.
Keywords:amygdala    ethanol    neuroactive steroids    neuropeptide Y    transgenic mice    Y1 receptor for neuropeptide Y
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