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Lipidic phase membrane protein serial femtosecond crystallography |
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| Authors: | Johansson Linda C Arnlund David White Thomas A Katona Gergely Deponte Daniel P Weierstall Uwe Doak R Bruce Shoeman Robert L Lomb Lukas Malmerberg Erik Davidsson Jan Nass Karol Liang Mengning Andreasson Jakob Aquila Andrew Bajt Saša Barthelmess Miriam Barty Anton Bogan Michael J Bostedt Christoph Bozek John D Caleman Carl Coffee Ryan Coppola Nicola Ekeberg Tomas Epp Sascha W Erk Benjamin Fleckenstein Holger Foucar Lutz Graafsma Heinz Gumprecht Lars Hajdu Janos Hampton Christina Y Hartmann Robert Hartmann Andreas Hauser Günter Hirsemann Helmut Holl Peter Hunter Mark S Kassemeyer Stephan |
| Affiliation: | Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden. |
| Abstract: | X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet. |
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