Isolation,crystallization and preliminary crystallographic data of aspartate aminotransferase from chicken heart mitochondria

The mitochondrial isoenzyme of aspartate aminotransferase (E.C. 2.6.1.1) has been isolated from chicken heart in an electrophoretically and immunologically homogeneous form. Large, well-diffracting single crystals of this enzyme, a dimeric molecule with a molecular weight of 90,000, have been grown by vapour phase diffusion against polyethylene glycol solutions. The crystals belong to space group P1. The unit cell, with the dimensions $\text{a = 55.6}\text{A}\text{?}\text{, 6 = 58.7}\text{A}\text{?}\text{, c = 76.0}\text{A}\text{?}$, α = 85.3 °, β = 109.2 °, γ = 115.6 °, contains a single dimer. The diffraction pattern extends to at least 2.1 Å resolution.