Identification of the structural gene for a myosin heavy-chain in Caenorhabditis elegans |
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Authors: | AR Macleod RH Waterston RM Fishpool S Brenner |
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Institution: | Medical Research Council Laboratory of Molecular Biology Hills Road, Cambridge, England |
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Abstract: | Mutants in the unc-54 gene of Caenorhabditis elegans have been characterized by cyanylation and sodium dodecyl sulphate/polyacrylamide gel electrophoresis of the total myosin present in each mutant. In the recessive mutants lacking a major fraction of the total myosin, the high molecular weight doublet of 15 × 104 and 14 × 104 which dominates the cyanylation pattern of the total wild-type myosin is absent. In the mutant E675, which possesses a novel heavy-chain with a molecular weight of 2 × 105, each component of the cyanylation doublet is reduced by 104 daltons, indicating that the doublet is derived from partial cleavage of a single polypeptide chain. This suggests that unc-54 is the structural gene for a myosin heavy-chain present in a major fraction of the total nematode myosin. |
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