Generation and characterization of transgenic poplar plants overexpressing a cotton laccase gene |
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Authors: | Ji Wang Chenglong Wang Mulan Zhu Yang Yu Yuebo Zhang Zhiming Wei |
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Institution: | (1) Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China;(2) Graduate School of the Chinese Academy of Sciences, Shanghai, 200032, China;(3) Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China |
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Abstract: | Laccases are copper-containing glycoproteins, which are widespread in higher plants as multigene families. To gain more insight
in the function of laccases in plants, especially potential role in lignification, we produced transgenic poplar plants overexpressing
a cotton laccase cDNA (GaLAC1) under the control of the cauliflower mosaic virus 35S promoter. As compared with untransformed control plants, transgenic
plants exhibited a 2.1- to 13.2-fold increased laccase activity, whereas plant growth rate and morphological characters remained
similar to control plants. A 2.1–19.6% increase in total lignin content of the stem was found in transgenic plants. Moreover,
transgenic plants showed a dramatically accelerated oxidation rate of phenolics, without obvious change in total phenolic
content. Our data suggested that GaLAC1 may participate in lignin synthesis and phenolic metabolism in plants. The present work provided a new genetic evidence for
the involvement of plant laccases in lignification. |
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Keywords: | Laccase Lignin Phenolic metabolism Populus Transgenic poplars |
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