正加速度暴露对大鼠海马星形胶质细胞GFAP表达的影响

目的探讨正加速度( Gz)重复暴露后不同时间海马星形胶质细胞GFAP表达的变化.方法 SD大鼠60只,随机分成对照组、 Gz重复暴露后1h、6h、12h、24h和48h组,每组10只.采用动物离心机,建立 Gz引发急性脑缺血模型;应用免疫组织化学技术,分别检测 Gz重复暴露后不同时间,海马星形胶质细胞GFAP的表达状况.结果海马星形胶质细胞GFAP阳性细胞数,在 Gz暴露后1h即显著增加,于12h达到高峰,而后逐渐下降,48h仍维持在较高水平,实验组与对照组比较,有显著性差异.结论 Gz重复暴露导致海马星形胶质细胞GFAP表达上调,可能对神经元的缺血损伤起保护作用.

EFFECT OF +Gz EXPOSURE ON GFAP EXPRESSION IN RAT HIPPOCAMPAL ASTROCYTES

Objective To detect the expression of glial fibrillary acidic protein(GFAP)in hippocampal astrocytes after +Gz exposures at different times.Methods Sixty conscious SD rats were randomly divided into six groups with 10 rats each: control,1h,6h,12h,24h and 48h groups after +Gz exposures.By means of an animal centrifuge,we set up a model of acute brain ischemia resulting from +Gz exposures,using immunohistochemistry after repeated +Gz exposuresto detect the expression of GFAP in hippocampal astrocytes at different times.Results The number of GFAP positive cellswas increased at 1h after +Gz exposures,reached its peak at 12h,and then decreased slowly.It was still sustained at a high level 48h later.It showed markedly differences compared with the controlgroup.Conclusion This study demonstrates that the expression of GFAP increases in hippocampal astrocytes after +Gz exposures,which may play a protective role for the neurons injured by ischemia.