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Establishment of a continuous cell line from fibrotic schistosomal granulomas in mice livers |
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| Authors: | Radovan Borojevic Alvaro N A Monteiro Solange A Vinhas Gilberto B Domont Paulo A S Mourão Hervé Emonard Gabriel Grimaldi Jr Jean-Alexis Grimaud |
| Institution: | 1. Institut Pasteur, CNRS ERA 819, 77 Rue Pasteur, 69007, Lyon, France 2. Departamento de Bioquimica, Instituto de Quimica, Universidade Federal do Rio de Janeiro, 21941 Ihla do Fund?o, Rio de Janeiro, Brasil 3. Departamento de Bioquimica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, 21941 Ilha do Fund?o, Rio de Janeiro, Brasil 4. Centro de Microscopia Eletr?nica, Instituto Oswaldo Cruz, 21040, Rio de Janeiro, Brasil |
| Abstract: | Summary A continuous murine cell line (GRX) was obtained from fibrotic granulomas induced in C3H/HeN mice liver by experimental infection withSchistosoma mansoni. This anchorage-dependent line produces composite connective tissue/extracellular matrix, displays morphological characteristics of myofibroblasts, and can, under appropriate conditions, accumulate fat droplets. GRX cells produce viral particles of retrovirus type. We consider GRX cell line to be representative of liver connective tissue cells, responsible for fibroplasia in liver fibrotic and granulomatous reactions. This research was supported by Centre National de la Recherche Scientifique, France; Financiadora de Estudos e Projetos (FINEP), Brasil; and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brasil. |
| Keywords: | liver fibrosis granulomatous inflammation Schistosoma mansoni myofibroblasts Ito cells |
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