DNA fingerprinting in roe deer using the digoxigenated probe (GTG)5 |
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Authors: | G Mörsch F Leibenguth |
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Institution: | Department Of Genetics, Saarland University, Saarbrücken, Germany |
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Abstract: | The digoxigenin-labelled oligonucleotide (GTG)5 was used as a multilocus probe to detect hypervariable microsatellites in roe deer DNA digested with Hae III. The resulting fingerprints of 24 animals belonging to four subpopulations were characterized with regard to within-subpopulation as well as between-subpopulation similarity. The mean number of polymorphic fragments was 20 and the average band-sharing rate for unrelated animals 0.27. A mean probability of 91.5% for a fragment to be present in the heterozygous state was evaluated and the probabilities of identical band patterns in unrelated individuals were estimated to be in the range 1.3 times 10-16 - 2.5 times 10-18. Though band-sharing rates of animals belonging to different subpopulations (range 0.18-0.24) were lower than those of within-subpopulations, several measures of population subdivision and the genetic distance do not reveal a striking differentiation of the subpopulations studied. |
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Keywords: | DNA fingerprints microsatellites polymorphisms population genetics |
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