Sequence variability of simian immunodeficiency virus in a persistently infected rhesus monkey |
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Authors: | D P Burns R C Desrosiers |
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Affiliation: | New England Regional Primate Research Center, Harvard Medical School, Southborough, MA. |
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Abstract: | A juvenile rhesus monkey that was inoculated intravenously with molecularly cloned SIVmac239 became persistently infected. A modified polymerase chain reaction (PCR) procedure was used to specifically amplify full-length envelope (env) gene sequences from DNA extracted from peripheral blood mononuclear cells (PBMC), lymph node tissue, and cells infected with recovered virus at 69 and 93 weeks post-infection. Extensive sequence variability accumulated in vivo in spite of infection with molecularly cloned virus. In the central portion of env. sequence variability was largely confined to three discrete regions. |
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Keywords: | Evolution Antigenic drift Genetic change Lentivirus Envelope Primate |
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