| 酮基布洛芬拆分用酯酶产生菌的筛选及其催化特性 |
| |
| 引用本文： | 沈端,许建和,宫鹏飞,刘幽燕,武慧渊.酮基布洛芬拆分用酯酶产生菌的筛选及其催化特性[J].微生物学通报,2002,29(1):45-49. |
| |
| 作者姓名： | 沈端 许建和 宫鹏飞 刘幽燕 武慧渊 |
| |
| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237 |
| |
| 基金项目： | 国家自然科学基金资助项目（No.20176011）~~ |
| |
| 摘 要： | 从土壤中筛选获得一株可以高对映选择性水解酮基布洛芬乙酯的酵母KET4，经鉴定为芸苔丝孢酵母（Trichosporon brassicae）。研究了该菌的生长和产酶过程，考察了其静息细胞对酮基布洛芬乙酯水解的催化特性。用该菌催化酯水解时，转化率为41%时，产物的对映体过量值为91%，对映选择率达到45。
|
| 关 键 词： | 手性药物 酮基布洛芬 光学拆分 芸苔丝孢酵母 酯酶产生菌 筛选 催化特性 |
| 文章编号： | 0253-2654(2002)01-0045-05 |
| 修稿时间： | 2000年11月2日 |
ISOLATION OF AN ESTERASE PRODUCER TRICHOSPRON BRASSICAE AND ITS CATALYTIC PERFORMANCE IN KINETIC RESOLUION OF KETOPROFEN |
| |
| SHEN Duan,XU Jian-He,GONG Peng-Ri,LIU You-Yan,WU Hui-Yuan.ISOLATION OF AN ESTERASE PRODUCER TRICHOSPRON BRASSICAE AND ITS CATALYTIC PERFORMANCE IN KINETIC RESOLUION OF KETOPROFEN[J].Microbiology,2002,29(1):45-49. |
| |
| Authors: | SHEN Duan XU Jian-He GONG Peng-Ri LIU You-Yan WU Hui-Yuan |
| |
| Abstract: | A strain of yeast capable of hydrolyzing ethyl ester of racemic Ketoprofen with high enantioselectivity has been isolated from soil after two-step enrichment. The yeast was identified as Trichosporon brassicae. The process of growth and enzyme production was investigated. The catalytic performance of the resting cell of KET4 in kinetic resolution of Ketoprofen was also investigated. When the conversion of substrate reached 41% , enantiomeric excess of the (S) - Ketoprofen produced was 91 % , indicating a high enantiomeric ratio of 45. |
| |
| Keywords: | Chiral drag Ketoprofen Optical resolution Trichosporon brassicae Esterase |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
| 点击此处可从《微生物学通报》下载免费的PDF全文 |
