组胺对大鼠小脑皮层浦肯野细胞的兴奋作用

在离体大鼠小脑脑片上观察了组胺对小脑皮层第Ⅹ小叶浦肯野细胞的作用。组胺（３～１００μｍｏｌ／Ｌ）主要引起浦肯野细胞的兴奋反应（９４４％,５１／５４）,在少数细胞上也观察到组胺所引起的放电抑制现象（５６％,３／５４）。用低Ｃａ２＋／高Ｍｇ２＋人工脑脊液灌流脑片,不能取消浦肯野细胞对组胺的兴奋反应（ｎ＝４）。Ｈ２受体对抗剂ｒａｎｉｔｉｄｉｎｅ（０１～５μｍｏｌ／Ｌ）能够阻断浦肯野细胞对组胺的兴奋反应（ｎ＝２０）,而Ｈ１受体对抗剂ｔｒｉｐｒｏｌｉｄｉｎｅ（０５～５μｍｏｌ／Ｌ）不能够（ｎ＝９）或仅轻微地（ｎ＝４）阻断浦肯野细胞对组胺的兴奋反应。这些结果提示,组胺可能主要通过Ｈ２受体的介导对浦肯野细胞起兴奋性调节作用,下丘脑小脑组胺能神经通路可能参与了小脑的某些躯体的和非躯体的功能调节。

The excitatory effects of histamine on cerebellar cortical Purkinje cells in the rat

The effects of histamine on Purkinje cells (PCs) in lobule of cerebellar cortex were investigated in rat cerebellar slices. Histamine elicited PCs responses predominately excitatory (944%, 51/54), only few inhibitory (56%, 3/54). The excitatory response could not be abolished by perfusing the slices with low Ca2 /high Mg2 medium. The excitatory response of PCs to histamine could be blocked by histamine H2 receptor antagonist ranitidine, but not by H1 receptor antagonist triprolidine readily. These results suggest that histamine exerts an excitatory effect on the PCs via H2 receptors. Presumably, hypothalamocerebellar histaminergic fibers participate in the regulation of somatic and nonsomatic functions of the cerebellum.