Biotransformation of albendazole by Cunninghamella blakesleeana: effect of carbon and nitrogen source |
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Authors: | G S Prasad S Girisham S M Reddy K Srisailam |
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Institution: | (1) Department of Microbiology, Kakatiya University, Warangal, India;(2) Department of Pharmacy, Kakatiya University, Warangal, India;(3) Vaagdevi College of Pharmacy, Ramnagar, Hanamkonda, Warangal, 506001, AP, India |
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Abstract: | A filamentous fungus Cunninghamella blakesleeana was screened for its ability to biotransform the anthelmintic drug albendazole. The fungus produced three metabolites in
the presence of the carbon and nitrogen sources studied. The transformation was identified by HPLC and the structures of the
transformation products were assigned by LC-MS-MS analysis and on the basis of previous reports. The results indicated that
the fungus metabolized albendazole into albendazole sulfoxide (M1), albendazole sulfone (M2) and an N-methyl metabolite of albendazole sulfoxide (M3). The effect of carbon and nitrogen source on the biotransformation of albendazole
was significant. Among the carbon and nitrogen sources studied, fructose and urea respectively produced maximum extent of
biotransformation in terms of substrate depletion. Among the carbon sources studied, maltose produced a higher percentage
of M1 whereas M2 and M3 were produced to their maximum extent in presence of d-fructose in terms of metabolite per unit quantity of biomass. In the case of nitrogen sources, ammonium acetate, calcium
nitrate and barium nitrate produced maximum percentage of M1, M2 and M3 respectively. The results reveal that the carbon and
nitrogen source significantly influence the microbial transformation both qualitatively and quantitatively. |
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Keywords: | Albendazole Albendazole sulfone Albendazole sulfoxide Biotransformation Carbon source Cunninghamella blakesleeana Metabolites Microorganisms Nitrogen source |
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