Trypanosoma brucei: cis-aconitate and temperature reduction as triggers of synchronous transformation of bloodstream to procyclic trypomastigotes in vitro |
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| Authors: | J Czichos C Nonnengaesser P Overath |
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| Affiliation: | 1. Institute of Animal Physiology, Department of Veterinary Sciences, Ludwig-Maximilians-University, Munich, Germany;2. Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany;3. Research Unit for Protein Science, Helmholtz Zentrum München, German Research Center for Environmental Health GmbH, Neuherberg, Germany;4. Centre of Ophthalmology, University Medical Centre, Eberhard-Karls-University Tuebingen, Tuebingen, Germany;1. Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ RJ 21.941-902, Brazil;2. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ RJ 21.941-902, Brazil;3. Departamento de Biologia Animal, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ RJ 23.890-000, Brazil;4. Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ RJ 21.941-902, Brazil |
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| Abstract: | Synchronous transformation of the monomorphic Trypanosoma brucei 427 variant clone MITat 1.4 (117) from bloodstream to procyclic trypomastigotes was studied in modified minimum essential medium plus 15% inactivated horse serum. Repression of variant surface glycoprotein synthesis, subsequent morphological transformation, and growth of procyclic cells was triggered by the simultaneous action of two signals: a reduction in temperature from 37 to 27 C and the addition of cis-aconitate. Repression of variant surface glycoprotein synthesis initiated by these two signals is reversible during the first hours, but becomes irreversible after about 1 day. Thereafter, cells are committed to differentiation at 27 C. |
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