Certain chromosomal regions in Streptomyces glaucescens tend to carry amplifications and deletions |
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| Authors: | Mamoru Hasegawa, Gilberto Hintermann, Jean-Marc Simonet, Reto Crameri, Jacqueline Piret Ralf Hü tter |
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| Affiliation: | (1) Tokyo Research Laboratories, Kyowa Hakko Co., Ltd., 3-6-6 Asahicho, Machidashi, Tokyo, Japan;(2) Mikrobiologisches Institut, Eidgenössische Technische Hochschule, ETH-Zentrum, CH-8092 Zürich, Switzerland;(3) Laboratoire de Biologie et Génétique Moléculaire, Université de Paris-Sud, F-91405 Orsay, France;(4) Biogen, S.A., Routes des Acacias 46, CH-1227 Carouge, Switzerland |
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| Abstract: | Summary Streptomycetes are subject to a high degree of genetic instability. One manifestation of this phenomenon is the occurrence of tandemly reiterated DNA stretches within the chromosome. We describe the analysis of ten reiterated sequences observed in various ethidium bromide-treated streptomycin-sensitive and melanin-negative mutant strains of Streptomyces glaucescens. The repeated DNA units were 2.9 to 35 kb in lenght. No two sequences were identical. The amplified sequences occupied up to 45% of the total genomic DNA. Structural analysis of the cloned repeated DNA stretches by means of restriction enzymes and by cross hybridization revealed the presence of two chromosomal areas rich in DNA reiterations. In some cases reiterated regions were accompanied by nearby rearrangements. |
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