Cloning of restriction fragments of DNA from staphylococcal bacteriophage phi 11. |
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| Authors: | S Lfdahl J E Sjstrm and L Philipson |
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| Institution: | S Löfdahl, J E Sjöström, and L Philipson |
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| Abstract: | EcoRI fragments of Staphylococcus aureus bacteriophage phi 11 DNA were cloned in vector plasmid pSA2100 in S. aureus. The clones were analyzed in marker rescue experiments with suppressor- and temperature-sensitive mutants of phi 11 to correlate the genetic and physical map. Several mutants could be identified on the physical map, and a clone containing fragment EcoRI-B of phi 11 DNA expressed immunity to phage infection. In addition, it was found that recombinant plasmids containing phi 11 DNA sequences can be transferred by high-frequency transduction after phage phi 11 infection of host cells. |
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