Identification of random amplified polymorphic DNA (RAPD) markers highly linked to sex determination in the red alga Gracilaria gracilis |
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Authors: | Martinez E A Destombe C Quillet M C Valero M |
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Affiliation: | Laboratorie de Génetique et Evolution des Populations Végétales, UPRESA CNRS 8016, GDR 1002, FR 1818, Villeneuve dAscq Cedex, France. |
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Abstract: | The random amplified polymorphic DNA (RAPD) technique was employed in the haplo-diploid dioecious species Gracilaria gracilis to identify sex-linked PCR markers. Sixty-nine decamer oligonucleotide primers were tested on two bulks of DNA, one from five haploid males and the other from five haploid females. One of these primers (OPD13) generated a 430-bp fragment specific to males and a 620-bp fragment specific to females. The diploid individuals (tetrasporophytes) showed the co-occurrence of these two fragments. In order to verify the linkage between the sexual phenotypes and these markers, a progeny array of 59 haploid individuals (male and female) born on a diploid individual was analysed, in all of which the two markers produced by the OPD13 primer segregated perfectly with sex. |
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Keywords: | BSA Gracilaria gracilis haplo-diploidy RAPD Rhodophyta sex-linked markers |
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