Enhanced oxygen transfer rates in fermentation using soybean oil-in-water dispersions |
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Authors: | J L Rols G Goma |
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Institution: | (1) Department de Génie Biochimique et Alimentaire, UA CNRS 544, Institut National des Sciences Appliquées, Complexe Scientifique de Rangueil, 31077 Toulouse Cédex, France |
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Abstract: | Summary The effect of soybean oil on the volumetric oxygen transfer coefficient during the cultivation ofAerobacter aerogenes cells is presented. For our aeration-agitation conditions (0.278 vvm and 500 rpm), it has been demonstrated that the use 19% (v/v) of soybean oil enabled a 1.85-fold increase of thek
l a coefficient (calculated on a per liter aqueous phase basis). For smaller volumetric oil fractions,k
L a increased linearly with the oil loading. Because of the oxygen-vector properties of soybean oil, this oil is able to significantly increase thek
L a of a bioreactor.Nomenclature C*, C
saturation and actual dissolved oxygen concentrations respectively (g/m3)
- KLa
volumetric oxygen transfer coefficient (h–1)
- KLainitial
k
La measured before the oil addition (h–1)
- MO2
molar mass of oxygen (dalton)
- N
oxygen transfer rate (g/m3. h)
- PO2. PN2
partial pressures ofO
2 andN
2 in the gas (atm)
- PH2OT
partial pressure of water in air at the temperatureT (atm)
- PT
total pressure (atm)
- Q0
volumetric flow rate of outlet air before seeding (m3/h)
- Sp
spreading coefficient (dynes/cm)
- T
absolute temperature of outlet gas (K)
- Vi
volume of the liquidi in the fermentor (m3)
- VM
molar volume at 273 K and 1 atm (m3/mole)
- ij
interfacial tension betweeni andj componants (dynes/cm)
- v
volumetric fraction of the oil (v/v)
- G
gas
- O
oil
- W
water
- i
inlet
- o
outlet |
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Keywords: | |
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