| 肝癌组织中GD3合成酶的纯化 |
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| 引用本文： | 夏霞娟,谢天培.肝癌组织中GD3合成酶的纯化[J].生物化学与生物物理学报,1994,26(4):351-356. |
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| 作者姓名： | 夏霞娟 谢天培 |
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| 作者单位： | 上海医科大学基础医学院生物化学教研室 |
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| 摘 要： | ＧＤ３合成酶是膜嵌合蛋白，定位于高尔基体，经过制作微粒体，Ｔｒｉｔｏｎ增溶、ＡＨ－Ｓｅｐｈａｒｏｓｅ及ＧＭ３－Ｇｌａｓｓｂｅａｄｓｅ及ＧＭ３－Ｇｌａｓｓｂｅａｄｓ亲和层析等步骤，二乙基亚硝胺诱发大鼠肝癌组织中的ＳＴ２被纯化了３１５９７倍，得率为０．３５％。ＳＤＳ－聚丙烯酰胺凝胶电泳后银染色呈１条蛋白着色带，分子量为５５ｋｄ。
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| 关 键 词： | GD3 合成酶 肝肿瘤 神经节苷酯 |
Purification of GD3 Synthetase from Rat Liver Cancer |
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| XIA Xia-Juan,XIE Tian-Pei and GU Tian-Jue.Purification of GD3 Synthetase from Rat Liver Cancer[J].Acta Biochimica et Biophysica Sinica,1994,26(4):351-356. |
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| Authors: | XIA Xia-Juan XIE Tian-Pei and GU Tian-Jue |
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| Abstract: | GD3 synthetase(ST2)is a membrane protein localized in the Golgi body.This paper reported its purification to homogeneity on SDS-PAGE. Liver cancer tissue from diethylnitrosamine-induced rat was homogenized and centrifuged.The membrane precipitation was solubilized with 1% Triton X-100/Triton CF-54(W/W1:1). After aminohexyl-Sepharose and GM3-Glassbeads affinity chromatography, the enzyme was purified by 31597 times. Its yield was 0.35%.Thepurified product gave one band on SDS-PAGE with silver staining with a MW of55kd. |
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| Keywords: | Liver cancer GD3 synthetase Purification |
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