人参皂苷Rg1对抑郁症大鼠抑郁行为和海马神经元损伤、PKA、PKC的影响

摘要 目的：探讨与分析人参皂苷Rg1对抑郁症大鼠抑郁行为和海马神经元损伤、蛋白激酶A（PKA）与蛋白激酶C（PKC）的影响。方法：抑郁症大鼠48只随机平分为三组-模型组、实验1组、实验2组，每组16只大鼠。实验1组、实验2组每天2次灌胃给药（1 mg/mL、4 mg/mL人参皂苷Rg1），给药体积为10 mL；模型组以相同方式按体重给予双蒸水。观察与记录鼠抑郁行为和海马神经元损伤、PKA、PKC表达变化情况。结果：实验1组、实验2组治疗第7 d、第14 d的逃避潜伏期都显著低于模型组，实验2组与实验1组相比也显著缩短（P<0.05）。实验1组、实验2组治疗第7 d、第14 d的糖水偏好率高于模型组，实验2组与实验1组相比也显著升高（P<0.05）。实验1组、实验2组治疗第7 d、第14 d的血清5-羟色胺较模型组高，血清皮质酮含量较模型组低，实验2组与实验1组对比也有明显差异（P<0.05）。实验1组、实验2组治疗第7 d、第14 d的海马神经元组织的PKA、PKC蛋白相对表达水平显著低于模型组，实验2组与实验1组相比也显著缩短（P<0.05）。结论：人参皂苷Rg1在抑郁症大鼠的应用能改善抑郁行为，增加糖水偏好率，降低逃避潜伏期，还可提高大鼠的血清5-羟色胺含量，降低血清皮质酮含量，降低海马神经元组织的PKA、PKC蛋白表达水平。

Effects of Ginsenoside Rg1 on Depression Behavior, Hippocampal Neuron Damage, PKA and PKC in Rats with Depression

ABSTRACT Objective: To investigate and analysis the effects of ginsenoside Rg1 on depression behavior, hippocampal neuron damage, protein kinase A (PKA) and protein kinase C (PKC) in rats with depression. Methods: 48 cases of rats with depression were randomly divided into three groups: model group, experimental group 1 and experimental group 2, with 16 rats in each group. Experiment group 1 and experiment group 2 were administered twice a day by gavage (1 mg/mL, 4 mg/ml ginsenoside Rg1) with a dose volume of 10ml; The model group were given double distilled water according to body weight in the same way. The depression behavior, hippocampal neuron injury, PKA and PKC expression were observed and recorded. Results: The escape latency of experimental group 1 and experimental group 2 on the 7th and 14th day of treatment were lower than that of model group, and the escape latency of experimental group 2 were also significantly shorter than that of experimental group 1 (P<0.05). The sugar preference rate of experimental group 1 and experimental group 2 on the 7th and 14th day of treatment were higher than that of model group, and the sugar preference rate of experimental group 2 were also higher than that of experimental group 1 (P<0.05). The serum 5-hydroxytryptamine level of experimental group 1 and experimental group 2 on the 7th and 14th day of treatment were higher than that of model group, and the serum corticosterone level were significantly lower than that of model group. There were also difference compared between experimental group 2 and experimental group 1(P<0.05). The relative expression levels of PKA and PKC protein in the hippocampal neurons of the experimental group 1 and the experimental group 2 on the 7th and 14th day of treatment were lower than that of the model group, and the experimental group 2 were also shorter than that of the experimental group 1(P<0.05). Conclusion: The application of ginsenoside Rg1 in rats with depression can improve the depressive behavior, increase the preference rate of sugar water, reduce the escape latency, increase the content of serum serotonin, reduce the content of serum corticosterone, and reduce the level of PKA and PKC protein expression in hippocampal neurons.