首页 | 本学科首页   官方微博 | 高级检索  
   检索      

深度突变扫描技术在蛋白研究中的应用
引用本文:李怡凡,王怡,张凯丽,李帅.深度突变扫描技术在蛋白研究中的应用[J].生物工程学报,2023,39(9):3710-3723.
作者姓名:李怡凡  王怡  张凯丽  李帅
作者单位:天津医科大学肿瘤医院乳腺病理研究室 国家恶性肿瘤临床医学研究中心 天津市恶性肿瘤临床医学研究中心 天津市"肿瘤防治"重点实验室 乳腺癌防治教育部重点实验室, 天津 300060
基金项目:国家自然科学基金(31870860);天津市医学重点学科(专科)建设项目(TJYXZDXK-012A)
摘    要:作为细胞结构与功能的中心参与者,蛋白质一直是生命科学研究的中心主题。分析蛋白质序列变异对其结构、功能的影响,是研究蛋白的重要手段之一。近年一种称为深度突变扫描(deep mutational scanning,DMS)的技术被广泛应用于蛋白研究领域,其通过高丰度DNA文库在蛋白特定区域平行引入成千上万种突变,经筛选后,利用高通量测序为每一种突变打分,从而揭示序列与功能之间的相关性。深度突变扫描以其高通量、快速简易、节省人工等特点,已经成为蛋白质功能研究以及蛋白工程改造的一种重要方法,目前已在蛋白进化、抗体改造、致病突变鉴定等蛋白研究的多个领域广泛应用。本综述简要概括了深度突变扫描技术的原理,重点介绍了其在哺乳动物细胞中的应用,同时分析了目前的技术瓶颈,旨在为相关研究提供参考。

关 键 词:深度突变扫描  文库构建  高通量测序  哺乳动物细胞
收稿时间:2022/12/30 0:00:00

Application of deep mutational scanning technology in protein research
LI Yifan,WANG Yi,ZHANG Kaili,LI Shuai.Application of deep mutational scanning technology in protein research[J].Chinese Journal of Biotechnology,2023,39(9):3710-3723.
Authors:LI Yifan  WANG Yi  ZHANG Kaili  LI Shuai
Institution:Key Laboratory of Breast Cancer Prevention and Therapy, Ministry of Education, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin''s Clinical Research Center for Cancer, Department of Breast Cancer Pathology and Research Laboratory, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Tianjin 300060, China
Abstract:As central players in cellular structure and function, proteins have long been central themes in life science research. Analyzing the impact of protein sequence variation on its structure and function is one of the important means to study proteins. In recent years, a technology called deep mutational scanning (DMS) has been widely used in the field of protein research. It introduces thousands of mutations in parallel in specific regions of proteins through high-abundance DNA libraries. After screening, high-throughput sequencing is employed to score each mutation, revealing sequence-function correlations. Due to its high-throughput, fast and easy, and labor-saving features, DMS has become an important method for protein function research and protein engineering. This review briefly summarizes the principle of DMS technology, highlighting its applications in mammalian cells. Moreover, this review analyzes the current technical bottlenecks, aiming to facilitate relevant research.
Keywords:deep mutational scanning  library construction  high-throughput sequencing  mammalian cells
本文献已被 维普 等数据库收录!
点击此处可从《生物工程学报》浏览原始摘要信息
点击此处可从《生物工程学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号