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Arrangement of F-actin and microtubules in the pseudopodia ofCryptochlora perforans (Chlorarachniophyta)
Authors:Cornelia Dietz  Reinhard Schnetter
Affiliation:(1) Botanisches Institut I, Justus-Liebig-Universität, D-35390 Gießen, Federal Republic of Germany
Abstract:Summary The distribution of actin and the arrangement of microtubules within the filopodia of amoeboid stages of Chlorarachniophyta were studied inCryptochlora perforans by indirect immunofluorescence. Actin is located along the whole pseudopodium, but at different concentrations. Microtubules run like coiled cables throughout the length of the pseudopodium. At the leading edges the pseudopodium frequently appears fan-shaped and the microtubules then show a spread-out arrangement, but they do not reach the cytoplasm front. Colchicine inhibited particle motility in the filopodia. The particle transport seems to be insensitive to cytochalasin D, but cells contracted their filopodia.Dedicated to Prof. Dr. Dr. h.c. Eberhard Schnepf on the occasion of his retirement
Keywords:Chlorarachniophyta  Cryptochlora perforans  Cytoskeleton  Immunofluorescence  Inhibitors
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