A free interface diffusion technique for the crystallization of proteins for x-ray crystallography |
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| Authors: | F R Salemme |
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| Affiliation: | 2. Sapienza University of Rome, Rome, Italy;3. Technion, Haifa, Israel;4. Imperial College London, London, United Kingdom;5. Technical University of Munich, Munich, Germany;1. Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India;2. Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India;3. King George Medical University, Lucknow, Uttar Pradesh 226003, India;1. Faculty of Physics, University of Vienna, Boltzmanngasse 5, A-1090 Vienna, Austria;2. Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, H-1111 Budapest, Hungary;3. Department of Chemistry, Eszterházy Károly University, Leányka utca 6, H-3300 Eger, Hungary |
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| Abstract: | A free interface diffusion technique for the crystallization of proteins for X-ray crystallography is described. The procedures and apparatus are simple and allow experiments to be carried out routinely with small quantities of protein. Detailed procedures for the crystallization of cytochrome c2 (Rhodospirillum rubrum), cytochrome c′ (Rhodopseudomonas palustris), cytochrome c′ (R. rubrum), and glyceraldehyde 3-phosphate dehydrogenase (Palinurus) are described to exemplify the technique. |
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