Cloning, purification and characterization of a functional anthracycline glycosyltransferase |
| |
| Authors: | Sianidis Giorgos Wohlert Sven-Eric Pozidis Charalambos Karamanou Spyridoula Luzhetskyy Andriy Vente Andreas Economou Anastassios |
| |
| Institution: | Institute of Molecular Biology and Biotechnology, FORTH and Department of Biology, University of Crete, P.O. Box 1527, Iraklio-Crete 71110, Greece. |
| |
| Abstract: | We have cloned the gene that encodes a novel glucosyl transferase (AraGT) involved in rhamnosylation of the polyketide antibiotic Aranciamycin in Streptomyces echinatus. AraGT comprises two domains characteristic of bacterial glycosyltranferases. AraGT was synthesized in E. coli as a decahistidinyl-tagged polypeptide. Purified AraGT is dimeric, displays a T(mapp) of 30 degrees C and can glycosylate the aglycone of an Aranciamycin derivative as shown by liquid chromatography and mass spectrometry. The availability of functional AraGT will allow the generation Aranciamycin-based combinatorial libraries. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
