Affinity of 5-thio-L-fucose-containing Lewis X (LeX) trisaccharide analogs to anti-LeX monoclonal antibody |
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| Authors: | Tsuruta O Yuasa H Hashimoto H Kurono S Yazawa S |
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| Institution: | Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan. |
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| Abstract: | 5-Thiofucose-containing LeX trisaccharide analogs Gal beta(1,4)5SFuc alpha(1,3)]GlcNAc-OMe (2) and Gal beta(1,4)5SFuc beta(1,3)]GlcNAc-OMe (4) were synthesized via 5-thiofucosylation of methyl 2-azido-lactoside derivative 6 by the trichloroacetimidate method. Inhibitory activity of these analogs for the binding of LeX to anti-Lex antibody was evaluated by enzyme immunoassay, indicating that anti-LeX strictly recognizes alpha-configuration of the fucose moiety and its binding pocket includes no advantageous region, such as hydrophobic area, for recognizing the ring sulfur atom of 5-thiofucosyl LeX analog 2. |
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