DNA content of Ulva compressa (Ulvales, Chlorophyta) nuclei determined with laser scanning cytometry |
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| Authors: | Yayoi Kagami Mariko Fujishita Kazuyo Matsuyama-Serisawa Maki Yamamoto Kazuyoshi Kuwano Naotsune Saga Shigeyuki Kawano |
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| Institution: | Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan;Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology'Yokosuka, Kanagawa 237-0061, Japan;Department of Marine Science and Technology, Graduate School of Science and Technology, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan;Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minatocho, Hakodate 041-8611, Japan |
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| Abstract: | The green macroalgal genus Ulva (incl. Entemmorpha) contains economically valuable species, is of relevance for coastal management (green tides), and certain taxa serve as experimental organisms for fundamental research in green algae. The nuclear genome size of Ulva (Entemmorpha) compressa Linnaeus was measured in propidium iodide stained nuclei using laser scanning cytometry. Nuclei of fixed gametes yielded reproducible values, whereas nuclei extracted from multicellular gametophytes were unsuitable. With nuclei of Arabidopsis thaliana (L.) Heynh and Saccharomyces cerevisiae Hansen as references, the haploid nuclear genome size of U. compressa was calculated as 135 ± 7 Mbp. This is the smallest genome so far known from any species of Ulva. |
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| Keywords: | gamete genome size green algae laser scanning cytometry nuclear DNA content Ulva compressa |
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