Na,K-ATPase in several tissues of the rat: Tissue-specific expression of subunit mRNAs and enzyme activity |
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| Authors: | Gregory G. Gick Mary Ann Hatala David Chon Faramarz Ismail-Beigi |
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| Affiliation: | (1) Department of Biochemistry, State University of New York Health Science Center at Brooklyn, 11203 Brooklyn, New York;(2) Department of Medicine, College of Physicians and Surgeons, Columbia University, 10032 New York, New York |
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| Abstract: | Summary The relative contents of Na,K-ATPase subunit mRNAs in rat renal cortex; ventricular myocardium, skeletal muscle (hind limb), liver and brain (cerebrum) were measured. Expressed per unit DNA, mRNA 1 content was  2-fold greater in the kidney and brain as compared to either heart, skeletal muscle or liver. The hierarchy of mRNA2 expression was brain > skeletal muscle > heart, whereas mRNA3 was restricted to brain. Betal subunit mRNA content in both kidney and brain exceeded the abundance of liver mRNA 1 by 7-fold. In all tissues examined, the combined abundances of the alpha subunit mRNAs exceeded the content of mRNA1 The hierarchy of Na,K-ATPase activity expressed per unit. DNA was brain > kidney > skeletal muscle = heart > liver. The sum of mRNA as well as mRNA1 content, expressed per g of tissue, was highest in brain and kidney. A statistically significant correlation between mRNA1 content and Na,K-ATPase activity was evident. |
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| Keywords: | sodium-potassium pump messenger ribonucleic acid abundance Northern blot hybridization analysis |
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