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利用相互染色体涂色技术分析两株人肺腺癌细胞系(A549和GLC-82)的核型特征
引用本文:彭昆靖,王金焕,苏伟婷,王熙才,杨凤堂,佴文惠.利用相互染色体涂色技术分析两株人肺腺癌细胞系(A549和GLC-82)的核型特征[J].动物学研究,2010,31(2):113-121.
作者姓名:彭昆靖  王金焕  苏伟婷  王熙才  杨凤堂  佴文惠
作者单位:1. 中国科学院昆明动物研究所,遗传资源与进化国家重点实验室,云南,昆明,650223;中国科学院研究生院,北京,100049
2. 中国科学院昆明动物研究所,遗传资源与进化国家重点实验室,云南,昆明,650223
3. 昆明医学院第三附属医院,云南省肿瘤医院/省肿瘤研究所,云南,昆明,650118
4. Wellcome Trust Sanger Institute,Wellcome Trust Genome Campus.Hinxton,Cambridge,CB10 ISA,UK
摘    要:肺癌是全世界癌症死亡中的一个主要的原因。除吸烟外,一些肺癌患者的发病与氡气污染相关。该研究采用包括染色体分选、正向和反向染色体涂色技术,分析了两株肺腺癌细胞系A549和GLC-82的核型特征。A549和GLC-82细胞系都属于非小细胞肺癌细胞系,但诱因不同,后者来源于一个长期生活在氡气污染环境肺癌病人的癌组织。染色体涂色结果表明,这两株肺癌细胞系发生了复杂的染色体重排。在A549和 GLC-82细胞系中,除正常染色体拷贝数变化外,还分别存在13条和24条畸变染色体。约一半的畸变染色体是通过非相互易位形成的,其余的畸变染色体则是通过一些正常染色体的片段缺失或重复而产生的。尽管这两株肺癌细胞系都没有共同的畸变染色体, 但它们似共享两个染色体易位断裂点:HSA8q24和12q14。

关 键 词:肺腺癌细胞系  染色体分选  染色体涂色  细胞遗传学特征
收稿时间:2009-11-13

Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting
PENG Kun-Jing,WANG Jin-Huan,SU Wei-Ting,WANG Xi-Cai,YANG Feng-Tang,NIE Wen-Hui.Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting[J].Zoological Research,2010,31(2):113-121.
Authors:PENG Kun-Jing  WANG Jin-Huan  SU Wei-Ting  WANG Xi-Cai  YANG Feng-Tang  NIE Wen-Hui
Abstract:Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome painting was used to characterize karyotypes of two lung adenocarcinoma cell lines: A549 and GLC-82 with the latter line derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines. Thirteen and twenty-four abnormal chromosomes were identified in A549 and GLC-82 cell lines, respectively. Almost half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines.
Keywords:Lung adenocarcinoma cell lines  Chromosome sorting  Chromosome painting  Cytogenetic characterization
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