An altered adenylate cyclase in cdc35-1 cell division cycle mutant of yeast |
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Authors: | J Sy Y Tamai |
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Institution: | 1. University of Technology, Mauritius;2. University of Central Lancashire, England, United Kingdom;3. EarthSight Foundation, India |
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Abstract: | Adenylate cyclase activity was studied in Saccharomyces cerevisiae's cell division cycle (cdc) mutant 35-1. The temperature sensitive mutant cdc35-1 was previously mapped as an allele of cyr, the adenylate cyclase gene. However, the adenylate cyclase activities of membranes prepared from cdc35-1 were not thermosensitive. The adenylate cyclase activity of cdc35-1 was found to have an altered Mn2+ dependency and did not respond to Gpp(NH)p stimulation. These results suggest that cdc35-1 mutation may not be at the catalytic site but at a site where adenylate cyclase interacts with regulatory proteins. |
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