Unique Pattern of Gene Expression in the Erythroid Precursor Cells |
| |
Authors: | YUJI MISHINA TAKASHI KATO AKIO URABE FUMIMARO TAKAKU SHUNJI NATORI MASUO OBINATA |
| |
Affiliation: | Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo;Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo |
| |
Abstract: | Erythroid cells were fractionated by preformed Percoll density gradient from livers of 12.5 day old mouse fetuses. With combination of lysing of mature erythroid cells, the CFU-E (colony forming unit of erythroid) was enriched as high as 30% pure. The mRNA levels of the rt-genes previously cloned as genes expressed in the reticulocytes are estimated in the fractionated erythroid cells. These rt-genes show a drastic change in expression during erythroid differentiation; Their expression was not detectable at the CFU-E cell stage. But it reached to maximum at the polychromatic erythroblast (stage I) and then decreases with maturation. The result suggests that mRNA synthesis of these rt-genes may be induced after the stimulation of erythropoietin. |
| |
Keywords: | |
|
|