Effect of thidiazuron on bud development of Cymbidium sinense Willd in vitro

Seed-derived rhizomes of Cymbidium sinense Willdwere able to initiate shoot buds in the presence of0.01--1 mg/l thidiazuron (TDZ). However, TDZ retardedrhizome proliferation. The regenerated shoot budswhich had been cultured on basal medium with asupplement of 0.5 mg/l 1-naphthalene acetic acid (NAA)and 1 g/l active charcoal were able to form 10 cmplantlets within four months. Plantlets weretransplanted to sphagnum moss and cultured on a mistbench for six months and then planted into soil-filledpots and maintained in the greenhouse, where they grewwell. In the 2nd year, 9.1% of the potted plants borenormal flowers. In the 3rd year, each of them produced5--6 shoots about 43 cm in height and about 20 roots;73% of the potted plants flowered normally.